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验证数据展示

  • IF Staining of PC-3 using CL488-17826

    IF Staining of PC-3 using CL488-17826

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 SPERT antibody (CL488-17826) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SPERT Polyclonal antibody

SPERT Polyclonal Antibody for IF/ICC
Cat No. CL488-17826

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Spermatid-associated protein, Spermatid associated protein, spermatid associated, Protein chibby homolog 2, NURIT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 17826-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17826 targets SPERT in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 SPERT fusion protein Ag12200 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatid associated
别名 Spermatid-associated protein, Spermatid associated protein, spermatid associated, Protein chibby homolog 2, NURIT
计算分子量 448 aa, 47 kDa, 52 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC027609
基因名称 SPERT
Gene ID (NCBI) 220082
RRIDAB_3672683
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NA61
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SPERT antibody CL488-17826Download protocol
Standard Protocols
Click here to view our Standard Protocols