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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SPERT Polyclonal antibody

SPERT Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17826

Print datasheet

Synonyms

Spermatid-associated protein, Spermatid associated protein, spermatid associated, Protein chibby homolog 2, NURIT

验证数据展示

  • IF Staining of PC-3 using CL488-17826

    IF Staining of PC-3 using CL488-17826

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 SPERT antibody (CL488-17826) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 17826-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17826 targets SPERT in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen SPERT fusion protein Ag12200 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spermatid associated
Synonyms Spermatid-associated protein, Spermatid associated protein, spermatid associated, Protein chibby homolog 2, NURIT
Calculated Molecular Weight 448 aa, 47 kDa, 52 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC027609
Gene Symbol SPERT
Gene ID (NCBI) 220082
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NA61
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SPERT antibody CL488-17826Download protocol
Standard Protocols
Click here to view our Standard Protocols