验证数据展示
经过测试的应用
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| CoIP | See 1 publications below |
产品信息
16555-1-AP targets SPG11 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SPG11 fusion protein Ag9769 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spastic paraplegia 11 (autosomal recessive) |
| 别名 | KIAA1840, Spastic paraplegia 11 protein, SPATACSIN, SPG11 |
| 计算分子量 | 2443 aa, 279 kDa |
| GenBank蛋白编号 | BC024161 |
| 基因名称 | SPG11 |
| Gene ID (NCBI) | 80208 |
| RRID | AB_2878277 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96JI7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPG11 antibody 16555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. | ||
PLoS Genet A hereditary spastic paraplegia mouse model supports a role of ZFYVE26/SPASTIZIN for the endolysosomal system. | ||
Hum Mol Genet SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction. | ||
Neurobiol Dis Loss of spatacsin function alters lysosomal lipid clearance leading to upper and lower motor neuron degeneration.
| ||
Ann Clin Transl Neurol Lysosomal abnormalities in hereditary spastic paraplegia types SPG15 and SPG11.
|