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验证数据展示

  • WB analysis using 68871-4-Ig

    WB analysis using 68871-4-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68871-4-Ig (SPHK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SPHK2 Monoclonal antibody

SPHK2 Monoclonal Antibody for WB, ELISA
Cat No. 68871-4-Ig

产品说明书

CloneNo. 2B5G5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HT-29 cells, Caco-2 cells, Jurkat cells, MOLT-4 cells, MJ cells, J774A.1 cells, Rat brain tissue, Mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy SPHK2 Ready-To-Use IHC Kit. SPHK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68871-4-Ig targets SPHK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPHK2 fusion protein Ag10569 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sphingosine kinase 2
别名
计算分子量 618 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC010671
基因名称 SPHK2
Gene ID (NCBI) 56848
RRIDAB_3670443
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRA0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).

实验方案

Product Specific Protocols
WB protocol for SPHK2 antibody 68871-4-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols