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SPHK2 Monoclonal antibody, PBS Only

SPHK2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B5G5

Cat no : 68871-4-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 68871-4-Ig (same clone as 68871-4-PBS)

    WB analysis using 68871-4-Ig (same clone as 68871-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68871-4-Ig (SPHK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68871-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SPHK2 Ready-To-Use IHC Kit. SPHK2 primary antibody included.

产品信息

68871-4-PBS targets SPHK2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SPHK2 fusion protein Ag10569 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sphingosine kinase 2
Synonyms
Calculated Molecular Weight 618 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010671
Gene Symbol SPHK2
Gene ID (NCBI) 56848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRA0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).