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SPIN1 Monoclonal antibody

SPIN1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C5

Cat no : 68615-1-Ig

Print datasheet

Synonyms

OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1

验证数据展示

  • WB analysis using 68615-1-Ig

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68615-1-Ig

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, NCCIT cells, rabbit brain tissue, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68615-1-Ig targets SPIN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen SPIN1 fusion protein Ag33686 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name spindlin 1
Synonyms OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1
Calculated Molecular Weight 262 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC013571
Gene Symbol SPIN1
Gene ID (NCBI) 10927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y657
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

实验方案

Product Specific Protocols
WB protocol for SPIN1 antibody 68615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols