SPTBN1 Monoclonal antibody
SPTBN1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, rabbit, pig and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1E1D1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse cerebellum tissue, HepG2 cells, Jurkat cells, pig brain, rabbit brain, rat brain, mouse brain, rat cerebellum |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SPTBN1 Ready-To-Use IHC Kit. SPTBN1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67978-1-Ig targets SPTBN1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Cited Reactivity | human |
| Immunogen | SPTBN1 fusion protein Ag31713 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | spectrin, beta, non-erythrocytic 1 |
| Synonyms | Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1 |
| Calculated Molecular Weight | 2364 aa, 275 kDa |
| Observed Molecular Weight | 250-275 kDa |
| GenBank Accession Number | BC137282 |
| Gene Symbol | SPTBN1 |
| Gene ID (NCBI) | 6711 |
| RRID | AB_2918727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q01082 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPTBN1 antibody 67978-1-Ig | Download protocol |
| IHC protocol for SPTBN1 antibody 67978-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cancer Clinical significance of nonerythrocytic spectrin Beta 1 (SPTBN1) in human kidney renal clear cell carcinoma and uveal melanoma: a study based on Pan-Cancer Analysis |
