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SQLE Monoclonal antibody, PBS Only

SQLE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A2

Cat No : 67206-1-PBS

Print datasheet

Synonyms

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase

验证数据展示

  • WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

    WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

产品信息

67206-1-PBS targets SQLE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, pig
Immunogen SQLE fusion protein Ag3266 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name squalene epoxidase
Synonyms ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase
Calculated Molecular Weight 574 aa, 64 kDa
Observed Molecular Weight 50-64 kDa
GenBank Accession NumberBC017033
Gene Symbol SQLE
Gene ID (NCBI) 6713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14534
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)