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CoraLite® Plus 488-conjugated SQLE Polyclonal antibody
SQLE Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-3 cells |
For other applications, we recommend the unconjugated version of this antibody, 12544-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12544 targets SQLE in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | SQLE fusion protein Ag3266 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | squalene epoxidase |
| Synonyms | ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase |
| Calculated Molecular Weight | 574 aa, 64 kDa |
| Observed Molecular Weight | 50-64 kDa |
| GenBank Accession Number | BC017033 |
| Gene Symbol | SQLE |
| Gene ID (NCBI) | 6713 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14534 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SQLE antibody CL488-12544 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |