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P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (9)


WB, IP, IF/ICC, IHC, ELISA and More (1)



Cat No : 18420-1-AP

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P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62


Positive WB detected inHeLa cells, U2OS cells, HEK-293 cells, HepG2 cells, U-87 MG cells, Jurkat cells
Positive IP detected inHepG2 cells, U2OS cells
Positive IHC detected inhuman liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HepG2 cells, U2OS cells, Chloroquine treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.


Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


18420-1-AP targets P62,SQSTM1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, goat, chicken, goslings, monkey, rabbit, zebrafish, hamster, pig, duck
Immunogen P62,SQSTM1 fusion protein Ag13131 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sequestosome 1
Synonyms P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.



 P62 (ubiquitin-binding protein P62), also known as Sequestosome-1, is a multifunctional adaptor protein most widely known for its role as an autophagosome cargo protein (PMID: 8551575). P62 via specific interactions with polyubiquitylated target proteins induces their selective autophagy (PMID: 17580304). It also plays an important role in the regulation of the NFkB signaling pathway, senescence, cell differentiation, apoptosis, and immune responses (PMID: 26404812).

 What is the molecular weight of P62?

 The observed molecular weight of the protein can vary from as low as 8 kDa (for the smallest isoforms) to 48 kDa.

 What is the subcellular localization of P62?

 P62 is mainly localized in the cytoplasm; however, upon autophagy induction, e.g., via starvation or selective inhibitor treatment, it localizes in vesicular structures - autophagosomes.

 What is the tissue specificity of P62?

 It is ubiquitously expressed in various tissues.

 What is the function of P62 in the regulation of cell death and autophagy?

 It is a selective autophagy receptor that forms a bridge between polyubiquitylated cargo (via its UBA domains) and an autophagy modifier such as LC3 (via LIR domains) (PMIDs: 16286508, 20168092, 24128730, 28404643, 22622177). The process of selective autophagy is tightly regulated at many levels, including the posttranslational modifications (PTMs) of various proteins in the cascade, P62 among others (PMID: 29233872). P62 is involved in the regulation of cell death induction in response to various stimuli, e.g., via activation of caspase-8 at the autophagosome membrane (PMID: 29480462). In addition, P62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression.

 What is P62's involvement in disease?

 Mutations in P62 have been associated with the following diseases: sporadic and familial Paget's disease of bone, neurodegenerative diseases, diabetes, and obesity (PMID: 29480462). A growing number of reports suggest the implication of P62 in the induction of multiple cellular oncogenic transformations. Indeed, increased levels of P62 have been linked to tumor formation, cancer promotion, and resistance to therapy (PMID: 29738493). Moreover, P62 is an unfavorable prognostic marker in liver cancer. 


Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IHC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IP protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
FC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
Standard Protocols
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