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P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18420-1-AP

Print datasheet

Synonyms

P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse liver tissue, HepG2 cells, U-87 MG cells, rat brain, rat liver
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inStarvation treated HepG2 cells, Chloroquine treated HeLa cells, U2OS cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®595 and CoraLite®650 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 18420-1-AP is P62,SQSTM1 Fusion Protein expressed in E. coli.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Ae. aegypti, bovine, chicken, goat, goslings, hamster, monkey, Monopterus albus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 种属同源性预测
Full Name sequestosome 1
Synonyms P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
UniprotQ13501
Gene ID (NCBI) 8878
RRIDAB_10694431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

 P62 (ubiquitin-binding protein P62), also known as Sequestosome-1, is a multifunctional adaptor protein most widely known for its role as an autophagosome cargo protein (PMID: 8551575). P62 via specific interactions with polyubiquitylated target proteins induces their selective autophagy (PMID: 17580304). It also plays an important role in the regulation of the NFkB signaling pathway, senescence, cell differentiation, apoptosis, and immune responses (PMID: 26404812).

 What is the molecular weight of P62?

 The observed molecular weight of the protein can vary from as low as 8 kDa (for the smallest isoforms) to 48 kDa.

 What is the subcellular localization of P62?

 P62 is mainly localized in the cytoplasm; however, upon autophagy induction, e.g., via starvation or selective inhibitor treatment, it localizes in vesicular structures - autophagosomes.

 What is the tissue specificity of P62?

 It is ubiquitously expressed in various tissues.

 What is the function of P62 in the regulation of cell death and autophagy?

 It is a selective autophagy receptor that forms a bridge between polyubiquitylated cargo (via its UBA domains) and an autophagy modifier such as LC3 (via LIR domains) (PMIDs: 16286508, 20168092, 24128730, 28404643, 22622177). The process of selective autophagy is tightly regulated at many levels, including the posttranslational modifications (PTMs) of various proteins in the cascade, P62 among others (PMID: 29233872). P62 is involved in the regulation of cell death induction in response to various stimuli, e.g., via activation of caspase-8 at the autophagosome membrane (PMID: 29480462). In addition, P62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression.

 What is P62's involvement in disease?

 Mutations in P62 have been associated with the following diseases: sporadic and familial Paget's disease of bone, neurodegenerative diseases, diabetes, and obesity (PMID: 29480462). A growing number of reports suggest the implication of P62 in the induction of multiple cellular oncogenic transformations. Indeed, increased levels of P62 have been linked to tumor formation, cancer promotion, and resistance to therapy (PMID: 29738493). Moreover, P62 is an unfavorable prognostic marker in liver cancer. 


实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IHC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IP protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
FC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells.

Authors - Xiangbo Meng
humanWB

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
mouseIHC,IF

Acta Neuropathol

Amyloidogenic α-synuclein seeds do not invariably induce rapid, widespread pathology in mice.

Authors - Amanda N Sacino
humanIF

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou