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验证数据展示

  • WB analysis of mouse heart using 21399-1-AP

    WB analysis of mouse heart using 21399-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21399-1-AP

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HEK-293 using 21399-1-AP

    IF Staining of HEK-293 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HeLa using 21399-1-AP

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 21399-1-AP

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SR140 Polyclonal antibody

SR140 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21399-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

U2SURP, U2-associated protein SR140, U2 snRNP-associated SURP motif-containing protein, KIAA0332, 140 kDa Ser/Arg-rich domain protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, human brain tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, HEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21399-1-AP targets SR140 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SR140 fusion protein Ag16093 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 U2-associated SR140 protein
别名 U2SURP, U2-associated protein SR140, U2 snRNP-associated SURP motif-containing protein, KIAA0332, 140 kDa Ser/Arg-rich domain protein
计算分子量 1029 aa, 118 kDa
观测分子量 120-140 kDa
GenBank蛋白编号BC111692
基因名称 SR140
Gene ID (NCBI) 23350
RRIDAB_10863661
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SR140 antibody 21399-1-APDownload protocol
IHC protocol for SR140 antibody 21399-1-APDownload protocol
IF protocol for SR140 antibody 21399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols