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CoraLite® Plus 488-conjugated SR140 Polyclonal antibody

SR140 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21399

Print datasheet

Synonyms

KIAA0332, SR140, U2 associated protein SR140, U2 associated SR140 protein, U2SURP

验证数据展示

  • IF Staining of HepG2 using CL488-21399

    IF Staining of HepG2 using CL488-21399

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SR140 antibody (CL488-21399) at dilution of 1:200.

  • FC experiment of HeLa using CL488-21399

    FC experiment of HeLa using CL488-21399

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sr140 Polyclonal Antibody (CL488-21399)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 21399-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21399 targets SR140 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SR140 fusion protein Ag16093 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name U2-associated SR140 protein
Synonyms KIAA0332, SR140, U2 associated protein SR140, U2 associated SR140 protein, U2SURP
Calculated Molecular Weight 1029 aa, 118 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC111692
Gene Symbol SR140
Gene ID (NCBI) 23350
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15042
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SR140 antibody CL488-21399Download protocol
FC protocol for CL Plus 488 SR140 antibody CL488-21399Download protocol
Standard Protocols
Click here to view our Standard Protocols