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验证数据展示

  • IF Staining of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    IF Staining of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00408-2

    Cytometric bead array standard curve of MP00408-2

    Cytometric bead array standard curve of MP00408-2, SREBF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83403-2-PBS. Detection antibody: 83403-1-PBS. Standard: Ag28205. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00408-3

    Cytometric bead array standard curve of MP00408-3

    Cytometric bead array standard curve of MP00408-3, SREBF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83403-4-PBS. Detection antibody: 83403-1-PBS. Standard: Ag28205. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83403-1-RR

    Affinity and Kinetic Characterization of 83403-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

SREBF2 Recombinant antibody, PBS Only (Detector)

SREBF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83403-1-PBS

Print datasheet

CloneNo. 240451A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SREBF2 Ready-To-Use IHC Kit. SREBF2 primary antibody included.

产品信息

83403-1-PBS targets SREBF2 as part of a matched antibody pair:

MP00408-2: 83403-2-PBS capture and 83403-1-PBS detection (validated in Cytometric bead array)

MP00408-3: 83403-4-PBS capture and 83403-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SREBF2 fusion protein Ag28205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sterol regulatory element binding transcription factor 2
Synonyms SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2
Calculated Molecular Weight 124 kDa
GenBank Accession NumberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12772
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.