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SREBF2 Recombinant antibody

SREBF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240451A12

Cat no : 83403-1-RR

Print datasheet

Synonyms

SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2

验证数据展示

  • IF Staining of HepG2 using 83403-1-RR

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83403-1-RR

    IF Staining of HepG2 using 83403-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83403-1-RR

    FC experiment of HeLa using 83403-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83403-1-RR

    Affinity and Kinetic Characterization of 83403-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SREBF2 Ready-To-Use IHC Kit. SREBF2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83403-1-RR is SREBF2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human
Immunogen SREBF2 fusion protein Ag28205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sterol regulatory element binding transcription factor 2
Synonyms SREBP 2, SREBF 2, Processed sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, bHLHd2
Calculated Molecular Weight 124 kDa
GenBank Accession NumberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.

实验方案

Product Specific Protocols
IF protocol for SREBF2 antibody 83403-1-RRDownload protocol
FC protocol for SREBF2 antibody 83403-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols