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验证数据展示

  • IF Staining of HepG2 using CL488-66742

    IF Staining of HepG2 using CL488-66742

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated SRF antibody (CL488-66742, Clone: 1G8F4 ) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SRF Monoclonal antibody

SRF Monoclonal Antibody for IF/ICC
Cat No. CL488-66742

产品说明书

CloneNo. 1G8F4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC

别名

MCM1, Serum response factor, SRF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66742-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66742 targets SRF in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 SRF fusion protein Ag10386 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serum response factor (c-fos serum response element-binding transcription factor)
别名 MCM1, Serum response factor, SRF
计算分子量 508 aa, 52 kDa
GenBank蛋白编号BC048211
基因名称 SRF
Gene ID (NCBI) 6722
RRIDAB_2883378
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11831
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SRF antibody CL488-66742Download protocol
Standard Protocols
Click here to view our Standard Protocols