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验证数据展示

  • IF Staining of HepG2 using CL488-66742

    IF Staining of HepG2 using CL488-66742

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated SRF antibody (CL488-66742, Clone: 1G8F4 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SRF Monoclonal antibody

SRF Monoclonal Antibody for IF/ICC
Cat No. CL488-66742
CloneNo. 1G8F4

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Synonyms

MCM1, Serum response factor, SRF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRF Ready-To-Use IHC Kit. SRF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66742-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66742 targets SRF in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen SRF fusion protein Ag10386 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Synonyms MCM1, Serum response factor, SRF
Calculated Molecular Weight 508 aa, 52 kDa
GenBank Accession NumberBC048211
Gene Symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2883378
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11831
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SRF antibody CL488-66742Download protocol
Standard Protocols
Click here to view our Standard Protocols