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验证数据展示

  • WB analysis using 68548-1-Ig

    WB analysis using 68548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68548-1-Ig (Spermidine synthase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 68548-1-Ig

    IF Staining of RAW 264.7 using 68548-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Spermidine synthase antibody (68548-1-Ig, Clone: 2F4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Spermidine synthase Monoclonal antibody

Spermidine synthase Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68548-1-Ig

产品说明书

CloneNo. 2F4E10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inRAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68548-1-Ig targets Spermidine synthase in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Spermidine synthase fusion protein Ag33919 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 spermidine synthase
别名 PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1
计算分子量 302 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC000309
基因名称 Spermidine synthase
Gene ID (NCBI) 6723
RRIDAB_3085253
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19623
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

实验方案

Product Specific Protocols
WB protocol for Spermidine synthase antibody 68548-1-IgDownload protocol
IF protocol for Spermidine synthase antibody 68548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols