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验证数据展示

  • IF Staining of HepG2 using CL488-68548

    IF Staining of HepG2 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

  • IF Staining of RAW 264.7 using CL488-68548

    IF Staining of RAW 264.7 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Spermidine synthase Monoclonal antibody

Spermidine synthase Monoclonal Antibody for IF/ICC
Cat No. CL488-68548

产品说明书

CloneNo. 2F4E10

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC

别名

SRM, SPS1, SPDSY, PAPT, EC:2.5.1.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68548-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68548 targets Spermidine synthase in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Spermidine synthase fusion protein Ag33919 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 spermidine synthase
别名 SRM, SPS1, SPDSY, PAPT, EC:2.5.1.16
计算分子量 302 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC000309
基因名称 Spermidine synthase
Gene ID (NCBI) 6723
RRIDAB_3673024
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19623
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Spermidine synthase antibody CL488-68548Download protocol
Standard Protocols
Click here to view our Standard Protocols