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CoraLite® Plus 488-conjugated Spermidine synthase Monoclonal antibody

Spermidine synthase Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4E10

Cat no : CL488-68548

Print datasheet

Synonyms

SRM, SPS1, SPDSY, PAPT, EC:2.5.1.16

验证数据展示

  • IF Staining of HepG2 using CL488-68548

    IF Staining of HepG2 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

  • IF Staining of RAW 264.7 using CL488-68548

    IF Staining of RAW 264.7 using CL488-68548

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 Spermidine synthase antibody (CL488-68548, Clone: 2F4E10 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68548-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68548 targets Spermidine synthase in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Spermidine synthase fusion protein Ag33919 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name spermidine synthase
Synonyms SRM, SPS1, SPDSY, PAPT, EC:2.5.1.16
Calculated Molecular Weight 302 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC000309
Gene Symbol Spermidine synthase
Gene ID (NCBI) 6723
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19623
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Spermidine synthase antibody CL488-68548Download protocol
Standard Protocols
Click here to view our Standard Protocols