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SRP19 Recombinant antibody, PBS Only

SRP19 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241219E9

Cat no : 84061-3-PBS

Print datasheet

Synonyms

241219E9

验证数据展示

  • WB analysis using 84061-3-RR (same clone as 84061-3-PBS)

    WB analysis using 84061-3-RR (same clone as 84061-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84061-3-RR (SRP19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84061-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84061-3-RR (same clone as 84061-3-PBS)

    IF Staining of HeLa using 84061-3-RR (same clone as 84061-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SRP19 antibody (84061-3-RR, Clone: 241219E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84061-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84061-3-RR

    Affinity and Kinetic Characterization of 84061-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84061-3-RR against Human SRP19 were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 84061-3-PBS is SRP19 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRP19 fusion protein Ag8903 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal recognition particle 19kDa
Synonyms 241219E9
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC010947
Gene Symbol SRP19
Gene ID (NCBI) 6728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09132
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA.