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验证数据展示

  • IF Staining of A549 using CL488-66266

    IF Staining of A549 using CL488-66266

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 SRPX2 antibody (CL488-66266, Clone: 2A1D7 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SRPX2 Monoclonal antibody

SRPX2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66266

产品说明书

CloneNo. 2A1D7

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig, mouse

应用

IF/ICC

别名

BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66266-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66266 targets SRPX2 in IF/ICC applications and shows reactivity with human, pig, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, pig, mouse
免疫原 SRPX2 fusion protein Ag8084 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 sushi-repeat-containing protein, X-linked 2
别名 BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2
计算分子量 465 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC020733
基因名称 SRPX2
Gene ID (NCBI) 27286
RRIDAB_2883298
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60687
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex suggests it has an important role in the perisylvian region critical for language and cognitive development (PMID: 16497722).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SRPX2 antibody CL488-66266Download protocol
Standard Protocols
Click here to view our Standard Protocols