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验证数据展示

  • WB analysis using 30851-1-AP

    WB analysis using 30851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30851-1-AP (SSR3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30851-1-AP

    IF Staining of mouse brain using 30851-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SSR3 antibody (30851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SSR3 Polyclonal antibody

SSR3 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30851-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

TRAPG, TRAP gamma, Translocon-associated protein subunit gamma, SSR-gamma, SSR gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells, mouse testis tissue, rat testis tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30851-1-AP targets SSR3 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SSR3 fusion protein Ag28130 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal sequence receptor, gamma (translocon-associated protein gamma)
别名 TRAPG, TRAP gamma, Translocon-associated protein subunit gamma, SSR-gamma, SSR gamma
计算分子量 185 aa, 21 kDa
观测分子量19 kDa
GenBank蛋白编号BC017203
基因名称 SSR3
Gene ID (NCBI) 6747
RRIDAB_3086422
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UNL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian TRAP is a heterotetrameric membrane protein complex which comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. SSR3 is also known as translocon-associated protein subunit gamma (TRAPG). The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. SSR3 assumes a central position in the mammalian TRAP complex, binding to the ribosome on the cytosolic face of the ER membrane and coordinating the remaining TRAP subunits with the ribosome and the other translocon components.

实验方案

Product Specific Protocols
WB protocol for SSR3 antibody 30851-1-APDownload protocol
IF protocol for SSR3 antibody 30851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols