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IL-1RL1/ST2 Polyclonal antibody

IL-1RL1/ST2 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 30194-1-AP

Print datasheet

Synonyms

IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6

验证数据展示

  • WB analysis using 30194-1-AP

    WB analysis using 30194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30194-1-AP (IL-1RL1/ST2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 30194-1-AP

    FC experiment of HEK-293 using 30194-1-AP

    1x10^6 HEK-293 cells were surface stained with 0.4 ug IL-1RL1/ST2 Polyclonal antibody (30194-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (blue). Cells were not fixed.

经过测试的应用

Positive WB detected inHUVEC cells, human placenta tissue
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy IL1RL1/ST2 Ready-To-Use IHC Kit. IL1RL1/ST2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30194-1-AP targets IL-1RL1/ST2 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 1 receptor-like 1
Synonyms IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6
Calculated Molecular Weight 63 kDa, 37 kDa, 30 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC030975
Gene Symbol IL1RL1
Gene ID (NCBI) 9173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01638
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

实验方案

Product Specific Protocols
WB protocol for IL-1RL1/ST2 antibody 30194-1-APDownload protocol
FC protocol for IL-1RL1/ST2 antibody 30194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols