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验证数据展示

  • FC experiment of HEK-293 using CL594-30194

    FC experiment of HEK-293 using CL594-30194

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite®594 IL-1RL1/ST2 Polyclonal Antibody (CL594-30194)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated IL-1RL1/ST2 Polyclonal antibody

IL-1RL1/ST2 Polyclonal Antibody for FC (Intra)
Cat No. CL594-30194

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy IL1RL1/ST2 Ready-To-Use IHC Kit. IL1RL1/ST2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30194-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-30194 targets IL-1RL1/ST2 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 1 receptor-like 1
Synonyms IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6
Calculated Molecular Weight 63 kDa, 37 kDa, 30 kDa
GenBank Accession NumberBC030975
Gene Symbol IL1RL1
Gene ID (NCBI) 9173
RRIDAB_3673431
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01638
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

实验方案

Product Specific Protocols
FC protocol for CL594 IL-1RL1/ST2 antibody CL594-30194Download protocol
Standard Protocols
Click here to view our Standard Protocols