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STARD10 Polyclonal antibody

STARD10 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17048-1-AP

Print datasheet

Synonyms

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

验证数据展示

  • WB analysis using 17048-1-AP

    WB analysis using 17048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17048-1-AP (STARD10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17048-1-AP

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 17048-1-AP

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 17048-1-AP

    IP experiment of HepG2 using 17048-1-AP

    IP result of anti-STARD10 (IP:17048-1-AP, 4ug; Detection:17048-1-AP 1:300) with HepG2 cells lysate 3400 ug.

经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17048-1-AP targets STARD10 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen STARD10 fusion protein Ag10705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name StAR-related lipid transfer (START) domain containing 10
Synonyms Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC007919
Gene Symbol STARD10
Gene ID (NCBI) 10809
RRIDAB_2255485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y365
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

实验方案

Product Specific Protocols
WB protocol for STARD10 antibody 17048-1-APDownload protocol
IHC protocol for STARD10 antibody 17048-1-APDownload protocol
IP protocol for STARD10 antibody 17048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols