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CoraLite®555-conjugated STAT4 Monoclonal antibody

STAT4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4A8C9

Cat no : CL555-67568

Print datasheet

Synonyms

SLEB11, DPMC, 4A8C9

验证数据展示

  • IF Staining of HepG2 using CL555-67568

    IF Staining of HepG2 using CL555-67568

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 STAT4 antibody (CL555-67568, Clone: 4A8C9 ) at dilution of 1:200.

  • FC experiment of mouse Th1 using CL555-67568

    FC experiment of mouse Th1 using CL555-67568

    1X10^6 mouse Th1 cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human STAT4 (CL555-67568, Clone:4A8C9) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inmouse Th1 cells
Planning an IHC experiment? We recommend our IHCeasy STAT4 Ready-To-Use IHC Kit. STAT4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67568 targets STAT4 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen STAT4 fusion protein Ag19545 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 4
Synonyms SLEB11, DPMC, 4A8C9
Calculated Molecular Weight 748 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC031212
Gene Symbol STAT4
Gene ID (NCBI) 6775
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14765
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].

实验方案

Product Specific Protocols
IF protocol for CL555 STAT4 antibody CL555-67568Download protocol
Standard Protocols
Click here to view our Standard Protocols