验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66427 targets STAT5B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | STAT5B fusion protein Ag2709 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 5B |
| 别名 | STAT5A/B, STAT5, 1D9B11 |
| 计算分子量 | 787 aa, 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC020868 |
| 基因名称 | STAT5B |
| Gene ID (NCBI) | 6777 |
| ENSEMBL Gene ID | ENSG00000173757 |
| RRID | AB_2934462 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51692 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 STAT5B antibody CL488-66427 | Download protocol |
| FC protocol for CL Plus 488 STAT5B antibody CL488-66427 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |