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STAT6 Recombinant antibody

STAT6 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F19

Cat no : 82630-1-RR

Print datasheet

Synonyms

STAT6C, STAT6B, STAT 6, Signal transducer and activator of transcription 6, IL-4 Stat

验证数据展示

  • IHC staining of human colon cancer using 82630-1-RR

    IHC staining of human colon cancer using 82630-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • WB analysis using 82630-1-RR

    WB analysis using 82630-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82630-1-RR (STAT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 82630-1-RR

    IHC staining of human stomach using 82630-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of mouse spleen using 82630-1-RR

    IHC staining of mouse spleen using 82630-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of mouse spleen using 82630-1-RR

    IHC staining of mouse spleen using 82630-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82630-1-RR targets STAT6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Synonyms STAT6C, STAT6B, STAT 6, Signal transducer and activator of transcription 6, IL-4 Stat
Calculated Molecular Weight 94 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC075852
Gene Symbol STAT6
Gene ID (NCBI) 6778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42226
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

实验方案

Product Specific Protocols
WB protocol for STAT6 antibody 82630-1-RRDownload protocol
IHC protocol for STAT6 antibody 82630-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols