验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | human colon cancer tissue, human stomach tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82630-1-RR targets STAT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | signal transducer and activator of transcription 6, interleukin-4 induced |
| Synonyms | STAT6C, STAT6B, STAT 6, Signal transducer and activator of transcription 6, IL-4 Stat |
| Calculated Molecular Weight | 94 kDa |
| Observed Molecular Weight | 105-110 kDa |
| GenBank Accession Number | BC075852 |
| Gene Symbol | STAT6 |
| Gene ID (NCBI) | 6778 |
| RRID | AB_3086494 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STAT6 antibody 82630-1-RR | Download protocol |
| IHC protocol for STAT6 antibody 82630-1-RR | Download protocol |
| IF protocol for STAT6 antibody 82630-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |