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验证数据展示

  • IF Staining of HeLa using CL488-82630

    IF Staining of HeLa using CL488-82630

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 STAT6 antibody (CL488-82630, Clone: 4F19 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-82630

    FC experiment of HepG2 using CL488-82630

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated STAT6 Recombinant antibody (CL488-82630, Clone:4F19)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated STAT6 Recombinant antibody

STAT6 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82630

产品说明书

CloneNo. 4F19

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

STAT6C, STAT6B, STAT 6, Signal transducer and activator of transcription 6, IL-4 Stat

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82630-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82630 targets STAT6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 6, interleukin-4 induced
别名 STAT6C, STAT6B, STAT 6, Signal transducer and activator of transcription 6, IL-4 Stat
计算分子量 94 kDa
观测分子量105-110 kDa
GenBank蛋白编号BC075852
基因名称 STAT6
Gene ID (NCBI) 6778
RRIDAB_3673069
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42226
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STAT6 antibody CL488-82630Download protocol
Standard Protocols
Click here to view our Standard Protocols