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STEAP2 Recombinant antibody

STEAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240611E5

Cat no : 83611-3-RR

Print datasheet

Synonyms

STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

验证数据展示

  • IF Staining of HEK-293 using 83611-3-RR

    IF Staining of HEK-293 using 83611-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2-Specific antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 83611-3-RR

    WB analysis using 83611-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83611-3-RR (STEAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 83611-3-RR

    IF Staining of HEK-293 using 83611-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2 antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A549 using 83611-3-RR

    FC experiment of A549 using 83611-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HEK-293 using 83611-3-RR

    FC experiment of HEK-293 using 83611-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, U2OS cells
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83611-3-RR targets STEAP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name six transmembrane epithelial antigen of the prostate 2
Synonyms STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberNM_152999
Gene Symbol STEAP2
Gene ID (NCBI) 261729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NFT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

实验方案

Product Specific Protocols
WB protocol for STEAP2 antibody 83611-3-RRDownload protocol
IF protocol for STEAP2 antibody 83611-3-RRDownload protocol
FC protocol for STEAP2 antibody 83611-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols