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验证数据展示

  • IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2-Specific antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • WB analysis using 83611-3-RR (same clone as 83611-3-PBS)

    WB analysis using 83611-3-RR (same clone as 83611-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83611-3-RR (STEAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    IF Staining of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STEAP2 antibody (83611-3-RR, Clone: 240611E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83611-3-RR (same clone as 83611-3-PBS)

    FC experiment of A549 using 83611-3-RR (same clone as 83611-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    FC experiment of HEK-293 using 83611-3-RR (same clone as 83611-3-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug STEAP2-Specific Recombinant antibody (83611-3-RR, Clone:240611E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83611-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00600-3

    Cytometric bead array standard curve of MP00600-3

    Cytometric bead array standard curve of MP00600-3, STEAP2-Specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83611-3-PBS. Detection antibody: 83611-5-PBS. Standard: SY00725. Range: 0.039-10 ng/mL.

STEAP2 Recombinant antibody, PBS Only (Capture)

STEAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83611-3-PBS

产品说明书

CloneNo. 240611E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83611-3-PBS targets STEAP2 as part of a matched antibody pair:

MP00600-3: 83611-3-PBS capture and 83611-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 six transmembrane epithelial antigen of the prostate 2
别名 STEAP2-Specific, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2
计算分子量 56 kDa
观测分子量 48-52 kDa
GenBank蛋白编号NM_152999
基因名称 STEAP2
Gene ID (NCBI) 261729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NFT2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.