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验证数据展示

  • WB analysis using 83484-2-RR

    WB analysis using 83484-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83484-2-RR (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83484-2-RR

    FC experiment of HeLa using 83484-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STEAP3 Recombinant antibody (83484-2-RR, Clone:1N5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83484-2-RR

    Affinity and Kinetic Characterization of 83484-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83484-2-RR against Human STEAP3 were performed. The affinity constant is 0.89 nM.

STEAP3 Recombinant antibody

STEAP3 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83484-2-RR

产品说明书

CloneNo. 1N5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-12 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STEAP3 Ready-To-Use IHC Kit. STEAP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83484-2-RR targets STEAP3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 STEAP3 fusion protein Ag29528 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 STEAP family member 3
别名 hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2
计算分子量 488 aa, 55 kDa
观测分子量55 kDa
GenBank蛋白编号BC042150
基因名称 STEAP3
Gene ID (NCBI) 55240
RRIDAB_3671112
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ658P3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

实验方案

Product Specific Protocols
WB protocol for STEAP3 antibody 83484-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols