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STIM1 Polyclonal antibody

STIM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11565-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

stromal interaction molecule 1, STRMK, stim-1, STIM 1, IMD10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11565-1-AP targets STIM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 STIM1 fusion protein Ag2140 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stromal interaction molecule 1
别名 stromal interaction molecule 1, STRMK, stim-1, STIM 1, IMD10
计算分子量 685 aa, 77 kDa
观测分子量 77-90 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
RRIDAB_2302808
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

实验方案

Product Specific Protocols
WB protocol for STIM1 antibody 11565-1-APDownload protocol
IHC protocol for STIM1 antibody 11565-1-APDownload protocol
IF protocol for STIM1 antibody 11565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.

Authors - Ao Hu
  • KO Validated
humanFC,IF

J Immunother Cancer

PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.

Authors - Hannah S Newton
mouseWB, IF

Proc Natl Acad Sci U S A

STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.

Authors - Hengtao Zhang
human,mouseWB

Cell Mol Life Sci

STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons

Authors - Karolina Serwach
  • KD Validated
humanWB, IF

Int J Mol Sci

Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.

Authors - Daniela Mählich
humanWB,IF

Oncogenesis

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu
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