验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Jurkat cells |
| Positive FC detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-11565 targets STIM1 in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | STIM1 fusion protein Ag2140 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | stromal interaction molecule 1 |
| Synonyms | D11S4896E, GOK, STIM1, stromal interaction molecule 1 |
| Calculated Molecular Weight | 685 aa, 77 kDa |
| Observed Molecular Weight | 77-90 kDa |
| GenBank Accession Number | BC021300 |
| Gene Symbol | STIM1 |
| Gene ID (NCBI) | 6786 |
| RRID | AB_3084526 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13586 |
| Storage Buffer | PBS with 50% Glycerol, 0.03% Proclin300, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 STIM1 antibody CL555-11565 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |