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CoraLite® Plus 555-conjugated STIM1 Polyclonal antibody

STIM1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-11565

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Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1



经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 11565-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-11565 targets STIM1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen STIM1 fusion protein Ag2140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stromal interaction molecule 1
Synonyms D11S4896E, GOK, STIM1, stromal interaction molecule 1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight 77-90 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13586
Storage Buffer PBS with 50% Glycerol, 0.03% Proclin300, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 STIM1 antibody CL555-11565Download protocol
Standard Protocols
Click here to view our Standard Protocols