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STIM1 Monoclonal antibody
STIM1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
rat, human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1D3A3
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, Jurkat cells |
Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66189-1-Ig targets STIM1 in WB, IF, IHC, ELISA applications and shows reactivity with rat, human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | rat, human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | STIM1 fusion protein Ag17914 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | stromal interaction molecule 1 |
Synonyms | |
Calculated Molecular Weight | 685 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC021300 |
Gene Symbol | STIM1 |
Gene ID (NCBI) | 6786 |
RRID | AB_2881584 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13586 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for STIM1 antibody 66189-1-Ig | Download protocol |
IHC protocol for STIM1 antibody 66189-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Biomed Pharmacother Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.
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J Transl Med Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X | ||
Neurotoxicology STIM1 Mediates Methamphetamine-Induced Neuronal Autophagy and Apoptosis
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