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  • KD/KO Validated

STIM1 Monoclonal antibody

STIM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D3A3

Cat no : 66189-1-Ig

Print datasheet

Synonyms



经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66189-1-Ig targets STIM1 in WB, IF, IHC, ELISA applications and shows reactivity with rat, human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity rat, human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen STIM1 fusion protein Ag17914 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name stromal interaction molecule 1
Synonyms
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
RRIDAB_2881584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.

实验方案

Product Specific Protocols
WB protocol for STIM1 antibody 66189-1-IgDownload protocol
IHC protocol for STIM1 antibody 66189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y

Authors - Ilaria Nigro
ratWB,IF

Biomed Pharmacother

Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.

Authors - Fangfang Xu
  • KD Validated
mouseWB

J Transl Med

Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X

Authors - Giusy Pietrafesa
human,mouseWB,IF

Neurotoxicology

STIM1 Mediates Methamphetamine-Induced Neuronal Autophagy and Apoptosis

Authors - Qin Tian
  • KD Validated