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STIM1 Monoclonal antibody

STIM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66189-1-Ig

产品说明书

CloneNo. 1D3A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

STRMK, stim-1, STIM 1, IMD10, GOK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66189-1-Ig targets STIM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 STIM1 fusion protein Ag17914 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 stromal interaction molecule 1
别名 STRMK, stim-1, STIM 1, IMD10, GOK
计算分子量 685 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
RRIDAB_2881584
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.

实验方案

Product Specific Protocols
WB protocol for STIM1 antibody 66189-1-IgDownload protocol
IHC protocol for STIM1 antibody 66189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y

Authors - Ilaria Nigro
ratWB,IF

Biomed Pharmacother

Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.

Authors - Fangfang Xu
  • KD Validated
mouseWB

J Transl Med

Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X

Authors - Giusy Pietrafesa
human,mouseWB,IF

Neurotoxicology

STIM1 Mediates Methamphetamine-Induced Neuronal Autophagy and Apoptosis

Authors - Qin Tian
  • KD Validated
WB

J Dairy Sci

Fatty acids promote migration of CD4+ T cells through calcium release-activated calcium modulator ORAI1 sensitive glycolysis in dairy cows

Authors - Bingbing Zhang
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