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  • Featured Product
  • KD/KO Validated

STIM1 Monoclonal antibody, PBS Only

STIM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3A3

Cat no : 66189-1-PBS

Print datasheet

Synonyms

STRMK, stim-1, STIM 1, IMD10, GOK

验证数据展示

  • WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

产品信息

66189-1-PBS targets STIM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STIM1 fusion protein Ag17914 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name stromal interaction molecule 1
Synonyms STRMK, stim-1, STIM 1, IMD10, GOK
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
RRIDAB_2881584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.