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验证数据展示

  • WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    WB analysis of K-562 using 66189-1-Ig (same clone as 66189-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    IHC staining of human lung cancer using 66189-1-Ig (same clone as 66189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

STIM1 Monoclonal antibody, PBS Only

STIM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66189-1-PBS

产品说明书

CloneNo. 1D3A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

STRMK, stim-1, STIM 1, IMD10, GOK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66189-1-PBS targets STIM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STIM1 fusion protein Ag17914 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 stromal interaction molecule 1
别名 STRMK, stim-1, STIM 1, IMD10, GOK
计算分子量 685 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
RRIDAB_2881584
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13586
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.