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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    WB analysis using 81156-1-RR (same clone as 81156-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR (same clone as 81156-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR (same clone as 81156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 81156-1-RR (STIM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 81156-1-RR (same clone as 81156-1-PBS)

    IF Staining of HEK-293 using 81156-1-RR (same clone as 81156-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using STIM1 antibody (81156-1-RR, Clone: 1B13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81156-1-RR

    Affinity and Kinetic Characterization of 81156-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81156-1-RR against Human STIM1 were performed. The affinity constant is 0.844 nM.

STIM1 Recombinant antibody, PBS Only

STIM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81156-1-PBS

Print datasheet

CloneNo. 1B13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1B13, GOK, IMD10, STIM 1, stim-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

81156-1-PBS targets STIM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STIM1 fusion protein Ag2140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name stromal interaction molecule 1
Synonyms 1B13, GOK, IMD10, STIM 1, stim-1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight77 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.