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验证数据展示

  • WB analysis using 81156-1-RR

    WB analysis using 81156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 81156-1-RR (STIM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 81156-1-RR

    IF Staining of HEK-293 using 81156-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using STIM1 antibody (81156-1-RR, Clone: 1B13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81156-1-RR

    Affinity and Kinetic Characterization of 81156-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81156-1-RR against Human STIM1 were performed. The affinity constant is 0.844 nM.

STIM1 Recombinant antibody

STIM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 81156-1-RR

产品说明书

CloneNo. 1B13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1B13, GOK, IMD10, STIM 1, stim-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, LNCaP cells, Jurkat cells, K-562 cells, Raji cells, RAW 263.7 cells, HSC-T6 cells, C6 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81156-1-RR targets STIM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STIM1 fusion protein Ag2140 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stromal interaction molecule 1
别名 1B13, GOK, IMD10, STIM 1, stim-1
计算分子量 685 aa, 77 kDa
观测分子量77 kDa
GenBank蛋白编号BC021300
基因名称 STIM1
Gene ID (NCBI) 6786
RRIDAB_2923705
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.

实验方案

Product Specific Protocols
WB protocol for STIM1 antibody 81156-1-RRDownload protocol
IHC protocol for STIM1 antibody 81156-1-RRDownload protocol
IF protocol for STIM1 antibody 81156-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols