验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, LNCaP cells, Jurkat cells, K-562 cells, Raji cells, RAW 263.7 cells, HSC-T6 cells, C6 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81156-1-RR targets STIM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | STIM1 fusion protein Ag2140 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | stromal interaction molecule 1 |
| Synonyms | 1B13, GOK, IMD10, STIM 1, stim-1 |
| Calculated Molecular Weight | 685 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC021300 |
| Gene Symbol | STIM1 |
| Gene ID (NCBI) | 6786 |
| RRID | AB_2923705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STIM1 antibody 81156-1-RR | Download protocol |
| IHC protocol for STIM1 antibody 81156-1-RR | Download protocol |
| IF protocol for STIM1 antibody 81156-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
