验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells |
For other applications, we recommend the unconjugated version of this antibody, 81156-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81156 targets STIM1 in WB applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | STIM1 fusion protein Ag2140 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | stromal interaction molecule 1 |
| 别名 | D11S4896E, GOK, STIM1, stromal interaction molecule 1 |
| 计算分子量 | 685 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC021300 |
| 基因名称 | STIM1 |
| Gene ID (NCBI) | 6786 |
| RRID | AB_3673724 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13586 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 STIM1 antibody CL750-81156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |