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- KD/KO Validated
STIM2 Polyclonal antibody
STIM2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 21192-1-AP is STIM2 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | STIM2 fusion protein Ag15530 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | stromal interaction molecule 2 |
| Synonyms | KIAA1482, STIM2, stromal interaction molecule 2 |
| Calculated Molecular Weight | 754 aa, 85 kDa |
| Observed Molecular Weight | 85-115 kDa |
| GenBank Accession Number | BC146661 |
| Gene Symbol | STIM2 |
| Gene ID (NCBI) | 57620 |
| RRID | AB_10734322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IHC protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IP protocol for STIM2 antibody 21192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.
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Cell Mol Life Sci STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons
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Oncotarget The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.
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Neurobiol Dis Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI. | ||
Biochem J STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes. | ||
Cell Signal RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.
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