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验证数据展示

  • WB analysis using 66637-1-Ig

    WB analysis using 66637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66637-1-Ig

    WB analysis of HeLa using 66637-1-Ig

    WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.

  • IF Staining of NIH/3T3 using 66637-1-Ig

    IF Staining of NIH/3T3 using 66637-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse kidney using 66637-1-Ig

    IHC staining of mouse kidney using 66637-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK3 antibody included
  • IHC staining of mouse kidney using 66637-1-Ig

    IHC staining of mouse kidney using 66637-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK3 antibody included
  • Featured Product
  • KD/KO Validated

STK3 Monoclonal antibody

STK3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66637-1-Ig

Print datasheet

CloneNo. 1C11A4
Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Synonyms

MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66637-1-Ig targets STK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen STK3 fusion protein Ag2741 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Synonyms MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 56-63 kDa
GenBank Accession NumberBC010640
Gene Symbol STK3
Gene ID (NCBI) 6788
RRIDAB_2881996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STK3 antibody 66637-1-IgDownload protocol
IHC protocol for STK3 antibody 66637-1-IgDownload protocol
IF protocol for STK3 antibody 66637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Gen Subj

Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP

Authors - Ana Paula Zen Petisco Fiore
View Article
humanWB,IF

Oncogene

Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired kinase and cell growth suppressing activities

Authors - Ana Maria Rodrigues
View Article