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验证数据展示

  • IF Staining of NIH/3T3 using CL488-66637

    IF Staining of NIH/3T3 using CL488-66637

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 STK3 antibody (CL488-66637, Clone: 1C11A4 ) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-66637

    FC experiment of NIH/3T3 using CL488-66637

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK3 (CL488-66637, Clone:1C11A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated STK3 Monoclonal antibody

STK3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66637

Print datasheet

CloneNo. 1C11A4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66637-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66637 targets STK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen STK3 fusion protein Ag2741 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Synonyms MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 56-63 kDa
GenBank Accession NumberBC010640
Gene Symbol STK3
Gene ID (NCBI) 6788
RRIDAB_2934484
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13188
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
FC protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
Standard Protocols
Click here to view our Standard Protocols