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验证数据展示

  • IF Staining of NIH/3T3 using CL488-66637

    IF Staining of NIH/3T3 using CL488-66637

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 STK3 antibody (CL488-66637, Clone: 1C11A4 ) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-66637

    FC experiment of NIH/3T3 using CL488-66637

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STK3 (CL488-66637, Clone:1C11A4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated STK3 Monoclonal antibody

STK3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66637

产品说明书

CloneNo. 1C11A4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK3 Ready-To-Use IHC Kit. STK3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66637-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66637 targets STK3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 STK3 fusion protein Ag2741 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase 3 (STE20 homolog, yeast)
别名 MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1
计算分子量 491 aa, 56 kDa
观测分子量 56-63 kDa
GenBank蛋白编号BC010640
基因名称 STK3
Gene ID (NCBI) 6788
RRIDAB_2934484
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13188
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
FC protocol for CL Plus 488 STK3 antibody CL488-66637Download protocol
Standard Protocols
Click here to view our Standard Protocols