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验证数据展示

  • WB analysis using 83081-2-RR (same clone as 83081-2-PBS)

    WB analysis using 83081-2-RR (same clone as 83081-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83081-2-RR (STK39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 83081-2-RR (same clone as 83081-2-PBS)

    IF Staining of NIH/3T3 using 83081-2-RR (same clone as 83081-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00106-3

    Sandwich ELISA standard curve of MP00106-3

    Sandwich ELISA standard curve of MP00106-3, Human STK39 Recombinant Matched Antibody Pair - PBS only. 83081-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34503. 83081-1-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00106-3

    Sample test of MP00106-3

    The mean STK39 concentration was determined to be 2,623.5 pg/mL in HepG2 cell extract based on a 4.2 mg/mL extract load and 9,245.2 pg/mL in Jurkat cell extract based on a 4.6 mg/mL extract load.

  • IF Staining of mouse brain using 83081-2-RR (same clone as 83081-2-PBS)

    IF Staining of mouse brain using 83081-2-RR (same clone as 83081-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00106-2

    Cytometric bead array standard curve of MP00106-2

    Cytometric bead array standard curve of MP00106-2, STK39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83081-2-PBS. Detection antibody: 83081-4-PBS. Standard: Ag34503. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83081-2-RR

    Affinity and Kinetic Characterization of 83081-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83081-2-RR against Human STK39 were performed. The affinity constant is 2.52 nM.

STK39 Recombinant antibody, PBS Only (Capture)

STK39 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83081-2-PBS

Print datasheet

CloneNo. 230382G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83081-2-PBS targets STK39 as part of a matched antibody pair:

MP00106-2: 83081-2-PBS capture and 83081-4-PBS detection (validated in Cytometric bead array)

MP00106-3: 83081-2-PBS capture and 83081-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse, rat
Immunogen STK39 fusion protein Ag34503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Synonyms Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9
Calculated Molecular Weight59 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).