验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, MCF-7 cells, SH-SY5Y cells, HEK-293T cells, HepG2 cells, rat brain tissue |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83081-2-RR targets STK39 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | STK39 fusion protein Ag34503 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | serine threonine kinase 39 (STE20/SPS1 homolog, yeast) |
别名 | Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9 |
计算分子量 | 59 kDa |
观测分子量 | 62 kDa |
GenBank蛋白编号 | BC166614 |
基因名称 | STK39 |
Gene ID (NCBI) | 27347 |
RRID | AB_3670797 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9UEW8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).
实验方案
Product Specific Protocols | |
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WB protocol for STK39 antibody 83081-2-RR | Download protocol |
IF protocol for STK39 antibody 83081-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |