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验证数据展示

  • WB analysis using 83081-2-RR

    WB analysis using 83081-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83081-2-RR (STK39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 83081-2-RR

    IF Staining of NIH/3T3 using 83081-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of mouse brain using 83081-2-RR

    IF Staining of mouse brain using 83081-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83081-2-RR

    Affinity and Kinetic Characterization of 83081-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83081-2-RR against Human STK39 were performed. The affinity constant is 2.52 nM.

STK39 Recombinant antibody

STK39 Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 83081-2-RR

Print datasheet

CloneNo. 230382G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, SH-SY5Y cells, HEK-293T cells, HepG2 cells, rat brain tissue
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83081-2-RR targets STK39 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STK39 fusion protein Ag34503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Synonyms Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9
Calculated Molecular Weight59 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
RRIDAB_3670797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

实验方案

Product Specific Protocols
WB protocol for STK39 antibody 83081-2-RRDownload protocol
IF protocol for STK39 antibody 83081-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols