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验证数据展示

  • IF Staining of SH-SY5Y using CL488-67204

    IF Staining of SH-SY5Y using CL488-67204

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 STMN2 antibody (CL488-67204, Clone: 1F6C4 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL488-67204

    FC experiment of SH-SY5Y using CL488-67204

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated STMN2 Monoclonal antibody (CL488-67204, Clone:1F6C4)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated STMN2 Monoclonal antibody

STMN2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67204

产品说明书

CloneNo. 1F6C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67204-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67204 targets STMN2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 STMN2 fusion protein Ag28990 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stathmin-like 2
别名 SGC 10, SCGN10, SCGN 10, SCG10, SCG 10
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC006302
基因名称 STMN2
Gene ID (NCBI) 11075
RRIDAB_3672941
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93045
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STMN2 antibody CL488-67204Download protocol
FC protocol for CL Plus 488 STMN2 antibody CL488-67204Download protocol
Standard Protocols
Click here to view our Standard Protocols