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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated STOML2 Monoclonal antibody

STOML2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2E9

Cat no : CL488-60052

Print datasheet

Synonyms

EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2

验证数据展示

  • FC experiment of HeLa using CL488-60052

    FC experiment of HeLa using CL488-60052

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human STOML2 (CL488-60052, Clone:1A2E9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60052-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60052 targets STOML2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen STOML2 fusion protein Ag0363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name stomatin (EPB72)-like 2
Synonyms EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2
Calculated Molecular Weight 356 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC002442
Gene Symbol STOML2
Gene ID (NCBI) 30968
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UJZ1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 STOML2 antibody CL488-60052Download protocol
Standard Protocols
Click here to view our Standard Protocols