验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60052 targets STOML2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | STOML2 fusion protein Ag0363 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | stomatin (EPB72)-like 2 |
| 别名 | EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2 |
| 计算分子量 | 356 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC002442 |
| 基因名称 | STOML2 |
| Gene ID (NCBI) | 30968 |
| RRID | AB_3084133 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UJZ1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 STOML2 antibody CL488-60052 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |