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验证数据展示

  • WB analysis using 68496-1-Ig

    WB analysis using 68496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 68496-1-Ig

    WB analysis using 68496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • FC experiment of A431 using 68496-1-Ig

    FC experiment of A431 using 68496-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.8 ug STT3B Monoclonal antibody (68496-1-Ig, Clone:3B7A8) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

STT3B Monoclonal antibody

STT3B Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68496-1-Ig

产品说明书

CloneNo. 3B7A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68496-1-Ig targets STT3B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 STT3B fusion protein Ag30866 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
别名 STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
计算分子量 97 kDa
观测分子量 94 kDa
GenBank蛋白编号BC015880
基因名称 STT3B
Gene ID (NCBI) 201595
RRIDAB_3085207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TCJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity

实验方案

Product Specific Protocols
WB protocol for STT3B antibody 68496-1-IgDownload protocol
FC protocol for STT3B antibody 68496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols