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验证数据展示

  • WB analysis using 68407-1-Ig

    WB analysis using 68407-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68407-1-Ig (STUB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of MCF-7 using 68407-1-Ig

    IF Staining of MCF-7 using 68407-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using STUB1 antibody (68407-1-Ig, Clone: 2E6B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

STUB1 Monoclonal antibody

STUB1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68407-1-Ig

产品说明书

CloneNo. 2E6B9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

CHIP, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, Antigen NY-CO-7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68407-1-Ig targets STUB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 STIP1 homology and U-box containing protein 1
别名 CHIP, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, Antigen NY-CO-7
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_005861
基因名称 STUB1
Gene ID (NCBI) 10273
RRIDAB_3085124
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UNE7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

实验方案

Product Specific Protocols
WB protocol for STUB1 antibody 68407-1-IgDownload protocol
IF protocol for STUB1 antibody 68407-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols