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验证数据展示

  • IF Staining of MCF-7 using CL488-68407

    IF Staining of MCF-7 using CL488-68407

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 STUB1 antibody (CL488-68407, Clone: 2E6B9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated STUB1 Monoclonal antibody

STUB1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68407

产品说明书

CloneNo. 2E6B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

IF/ICC

别名

Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy STUB1 Ready-To-Use IHC Kit. STUB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68407-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68407 targets STUB1 in IF/ICC applications and shows reactivity with Human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 STIP1 homology and U-box containing protein 1
别名 Antigen NY CO 7, CHIP, CLL associated antigen KW 8, HSPABP2, NY CO 7, SDCCAG7, STUB1, UBOX1
计算分子量 35 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_005861
基因名称 STUB1
Gene ID (NCBI) 10273
RRIDAB_3084482
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UNE7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STUB1 antibody CL488-68407Download protocol
Standard Protocols
Click here to view our Standard Protocols