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验证数据展示

  • WB analysis using 81899-1-RR (same clone as 81899-1-PBS)

    WB analysis using 81899-1-RR (same clone as 81899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81899-1-RR (STX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    WB analysis of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    WB result of STX17 antibody (81899-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells. This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

    IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

    IHC staining of mouse kidney using 81899-1-RR (same clone as 81899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    IF Staining of HepG2 using 81899-1-RR (same clone as 81899-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STX17 antibody (81899-1-RR, Clone: 4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81899-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81899-1-RR

    Affinity and Kinetic Characterization of 81899-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81899-1-RR against Human STX17 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

STX17 Recombinant antibody, PBS Only

STX17 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81899-1-PBS

产品说明书

CloneNo. 4G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Syntaxin-17, Syntaxin 17, 4G3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81899-1-PBS targets STX17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STX17 fusion protein Ag12134 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 17
别名 Syntaxin-17, Syntaxin 17, 4G3
计算分子量 302 aa, 33 kDa
观测分子量 33-35 kD
GenBank蛋白编号BC111009
基因名称 STX17
Gene ID (NCBI) 55014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56962
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.