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验证数据展示

  • WB analysis using 83159-6-RR (same clone as 83159-6-PBS)

    WB analysis using 83159-6-RR (same clone as 83159-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 83159-6-RR (same clone as 83159-6-PBS)

    IF Staining of mouse cerebellum using 83159-6-RR (same clone as 83159-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

STX1A Recombinant antibody, PBS Only

STX1A Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83159-6-PBS

产品说明书

CloneNo. 230470F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83159-6-PBS targets STX1A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 1A (brain)
别名 Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号NM_004603
基因名称 STX1A
Gene ID (NCBI) 6804
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16623
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.