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验证数据展示

  • WB analysis using 83159-6-RR

    WB analysis using 83159-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83159-6-RR

    IHC staining of mouse brain using 83159-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83159-6-RR

    IHC staining of mouse brain using 83159-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 83159-6-RR

    IF Staining of mouse cerebellum using 83159-6-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STX1A Recombinant antibody

STX1A Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83159-6-RR

产品说明书

CloneNo. 230470F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, human brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83159-6-RR targets STX1A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syntaxin 1A (brain)
别名 Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号NM_004603
基因名称 STX1A
Gene ID (NCBI) 6804
RRIDAB_3670852
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16623
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.

实验方案

Product Specific Protocols
WB protocol for STX1A antibody 83159-6-RRDownload protocol
IHC protocol for STX1A antibody 83159-6-RRDownload protocol
IF protocol for STX1A antibody 83159-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols