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验证数据展示

  • WB analysis using 83159-6-RR

    WB analysis using 83159-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83159-6-RR

    IHC staining of mouse brain using 83159-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83159-6-RR

    IHC staining of mouse brain using 83159-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 83159-6-RR

    IF Staining of mouse cerebellum using 83159-6-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STX1A Recombinant antibody

STX1A Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83159-6-RR
CloneNo. 230470F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, human brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83159-6-RR targets STX1A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name syntaxin 1A (brain)
Synonyms Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberNM_004603
Gene Symbol STX1A
Gene ID (NCBI) 6804
RRIDAB_3670852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16623
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.

实验方案

Product Specific Protocols
WB protocol for STX1A antibody 83159-6-RRDownload protocol
IHC protocol for STX1A antibody 83159-6-RRDownload protocol
IF protocol for STX1A antibody 83159-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols