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STXBP1 Monoclonal antibody, PBS Only

STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B3

Cat no : 67137-1-PBS

Print datasheet

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

验证数据展示

  • WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

    WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

    FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

产品信息

67137-1-PBS targets STXBP1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen STXBP1 fusion protein Ag28605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_2882436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.