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STXBP1 Monoclonal antibody, PBS Only

STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5B3

Cat no : 67137-1-PBS

Print datasheet

Synonyms

N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4

验证数据展示

  • WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

    WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STXBP1 antibody included
  • IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

    FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STXBP1 Ready-To-Use IHC Kit. STXBP1 primary antibody included.

产品信息

67137-1-PBS targets STXBP1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen STXBP1 fusion protein Ag28605 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syntaxin binding protein 1
Synonyms N Sec1, Munc18-1, MUNC18 1, FLJ37475, EIEE4
Calculated Molecular Weight 594 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC015749
Gene Symbol STXBP1
Gene ID (NCBI) 6812
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61764
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.